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KMID : 1123920030170051330
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 5 p.1330 ~ p.1334
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The effect of Astragali Radix Ethanol extract on Murine CD4 T cells′ Cytokine Profiles in vitro
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Kang Hee
Bae Hyun-Su
Ahn Kyoo-Seok
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Abstract
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Astragali Radix(AR), one of the strong tonic herbs, is known to improve immunological responses in mice and human. In this study, AR’s ai-reinforcing effect was examined in the context of CD4/sup +/ T cells’ TCR/CD3 induced activation responses. In order to evaluate the direct effect of AR on helper T cells, CD4/sup +/ T cells are isolated using magnetic bead and their proliferation and CD69 expression in AR treated medium were assessed with anti-CD3/anti-CD28 activation for 48h. CD4 T cells’ proliferation was slightly increased but there was little effect on CD69 expression. RT PCR and ELISA equally demonstrated that IL-2 and IL-4 production was increased but IFN-ν was down-regulated. This shows AR ethanol extract favors Th2 cytokine profile under neutral conditions.
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KEYWORD
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Astragali Radix(AR), CD4 T cell, IL-2, IL-4, IFN-ν
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